Immunohistochemical Staining of MATR3 in Tumor Tissues

Immunohistochemical (IHC) staining was carried out on tissue macromatricescomposed of representative tumor areas. Five different large tissue fragments from donor paraffin blocks were present in one recipient block. The sections (4 μm thick) were cut from each tissue macroarray block and placed on high-adhesive glass slides (SuperFrost Plus; Menzel-Glaser, Braunschweig, Germany). The sections were subjected to IHC staining according to the previously described protocol [10 (link),11 (link)]. Immunohistochemistry was carried out by BenchMark® Ultra automatic staining machine (Roche Diagnostics/Ventana Medical Systems, Tucson, AZ, USA) with Ventana UltraView DAB Detection Kit (Ventana Medical Systems). MATR3 was detected by using anti-MATR3 rabbit polyclonal antibody (cat. no: HPA036565, Sigma-Aldrich, St. Louis, MO, USA) in a 1:300 dilution for 32 min.

Free full text: Click here

Durślewicz J., Klimaszewska-Wiśniewska A., Antosik P, & Grzanka D. (2023). Low Expression of MATR3 Is Associated with Poor Survival in Clear Cell Renal Cell Carcinoma. Biomedicines, 11(2), 326.

Publication 2023

Ventana UltraView DAB Detection Kit

Anti antibody Diagnostics Dilution Donor Immunohistochemistry Paraffin Rabbit Tissue Tumor

Corresponding Organization : Collegium Medicum in Bydgoszcz

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Immunohistochemical (IHC) staining on tissue macromatrices

dependent variables

MATR3 protein expression

control variables

Tissue macroarray blocks
High-adhesive glass slides (SuperFrost Plus)
Previously described IHC staining protocol [10, 11]
BenchMark® Ultra automatic staining machine
Ventana UltraView DAB Detection Kit
Anti-MATR3 rabbit polyclonal antibody (cat. no: HPA036565, Sigma-Aldrich) in a 1:300 dilution for 32 min

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!