Fluorescently Tagged HeLa Cell Line

HeLa cells with endogenous tagging with fluorescent proteins of β-tubulin (mClover3), histone H1 (mTagBFP2), and p62-SQSTM1 (mRuby3) were developed with CRISPR and the FAST-HDR vector system, and were recently described [17 (link)]. These cells are commercially available from ExpressCells, Inc. A clonal cell line was derived by single cell sorting with the Hana Single Cell Sorter Instrument (Namocell) using the green fluorescent channel and sorting into a 96 well plate. Single cell clones were analyzed with a Spark Cyto plate reader (Tecan) using whole-well imaging for selecting clones derived from a single cell. A clone with uniform and bright β-tubulin fluorescence was selected to expand a cell line for this study.

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Khachatryan H., Olszowy B., Barrero C.A., Gordon J, & Perez-Leal O. (2023). Identification of Inhibitors of Tubulin Polymerization Using a CRISPR-Edited Cell Line with Endogenous Fluorescent Tagging of β-Tubulin and Histone H1. Biomolecules, 13(2), 249.

Publication 2023

Spark Cyto

Cell Cell line Clone Crispr Fluorescence Hela cells Histone h1 Proteins Tubulin Vector

Corresponding Organization : Temple University

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Variable analysis

independent variables

Endogenous tagging with fluorescent proteins of β-tubulin (mClover3), histone H1 (mTagBFP2), and p62-SQSTM1 (mRuby3)

dependent variables

Uniform and bright β-tubulin fluorescence

control variables

CRISPR and the FAST-HDR vector system used for endogenous tagging
Clonal cell line derived by single cell sorting with the Hana Single Cell Sorter Instrument (Namocell)
Whole-well imaging with a Spark Cyto plate reader (Tecan) to select clones derived from a single cell

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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