Dimethyl sulfoxide (DMSO) was purchased from Sigma. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (>99% purity) was originally obtained from Dow Chemical Co. (Midland, MI, United States). 6-Formylindolo[3,2-b]carbazole (FICZ), Indole-3-carbinol.
(I3C), 12-O-tetradecanoylphorbol-13-acetate (TPA) and other molecular biological reagents were purchased from Cayman Chemicals (Ann Arbor, MI, United States) and Applied Biosystems (Foster City, CA, United States). TLR ligands were purchased from InvivoGen (San Diego, CA, United States). RelB-specific polyclonal antibody from Active Motif (Carlsbad, CA, United States). AhR-specific polyclonal antibody from Enzo Life Sciences (Farmingdale, NY, United States). The traffic-related air pollution (TRAP)-related PM2.5 was collected from an exposure facility immediately adjacent to a major freeway tunnel system in Northern California (Patten et al., 2020 (link)) via impaction-based filter sampling and extracted according to the protocols of Bein and Wexler (2014) (link) and Bein and Wexler (2015) (link). TRAP samples were collected before and during the Sonoma/Napa wildfire in 2017. The wildfire PM sample was collected during the Carr wildfire in Northern California in 2018. The dry PM extracts were resuspended in DMSO and sonicated immediately prior to treatment of the cells.
(I3C), 12-O-tetradecanoylphorbol-13-acetate (TPA) and other molecular biological reagents were purchased from Cayman Chemicals (Ann Arbor, MI, United States) and Applied Biosystems (Foster City, CA, United States). TLR ligands were purchased from InvivoGen (San Diego, CA, United States). RelB-specific polyclonal antibody from Active Motif (Carlsbad, CA, United States). AhR-specific polyclonal antibody from Enzo Life Sciences (Farmingdale, NY, United States). The traffic-related air pollution (TRAP)-related PM2.5 was collected from an exposure facility immediately adjacent to a major freeway tunnel system in Northern California (Patten et al., 2020 (link)) via impaction-based filter sampling and extracted according to the protocols of Bein and Wexler (2014) (link) and Bein and Wexler (2015) (link). TRAP samples were collected before and during the Sonoma/Napa wildfire in 2017. The wildfire PM sample was collected during the Carr wildfire in Northern California in 2018. The dry PM extracts were resuspended in DMSO and sonicated immediately prior to treatment of the cells.
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