Tissue was processed for immunoblotting as described (Kao and Bartel, 2015 (link)), except that protein transfer was for 50 min and membranes were air dried before blocking in 8% non-fat dry milk in TBST (Tris-buffered saline with 0.1% Tween-20). Primary antibodies were as follows: rabbit anti-PEX1 (1:200, Rinaldi et al., 2017 ), anti-PEX5 (1:100, Zolman and Bartel, 2004 (link)), anti-PEX6 (1:800–1000, Ratzel et al., 2011 (link)), anti-PEX7 (1:2000, Ramón and Bartel, 2010 (link)), anti-PEX10 (1:500, Burkhart et al., 2014 (link)), anti-PEX14 (1:10,000, Agrisera AS08), anti-thiolase (1:2,500-1:5000, Lingard et al., 2009 (link)), anti-PMDH2 (1:2,000, Pracharoenwattana et al., 2007 (link)); mouse anti-mitochondrial ATP synthase subunit α (1:2,000, MitoScience MS507) and anti-HSC70 (1:50,000–1:100,000, SPA-817, StressGen Biotechnologies); and rat anti-HA (1:100–1:500, Roche clone 3F10). Horseradish peroxidase-conjugated secondary antibodies (1:5,000) were incubated for 3–6 hours before washing and imaging using WesternBright ECL substrate (Advansta). Films were scanned with a flatbed scanner, and bands were quantified using NIH image J. Membranes were sequentially probed with various antibodies without stripping. Immunoblotting experiments were repeated at least twice with similar results.