Frankincense and Sandalwood Essential Oils in Cell Culture

Cell culture medium (MEM and DMEM/F-12 (1:1)), fetal bovine serum (FBS), MEM vitamin solution, non-essential amino acids, epidermal growth factor (EGF), insulin-transferrin-sodium selenite (ITS) media supplement, sodium pyruvate, and penicillin-streptomycin were purchased from Life Technologies (Grand Island, NY, USA). Frankincense (B. carterii from Somalia) and sandalwood (S. album from Sri Lanka) essential oils were obtained from Young Living Essential Oils (Lehi, UT, USA) and prepared based on previously reported procedures [7 (link)]. An XTT cell proliferation assay kit was obtained from Roche Applied Science (Indianapolis, IN, USA). An RNeasy® Mini Kit was obtained from Qiagen (Valencia, CA, USA).

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Dozmorov M.G., Yang Q., Wu W., Wren J., Suhail M.M., Woolley C.L., Young D.G., Fung K.M, & Lin H.K. (2014). Differential effects of selective frankincense (Ru Xiang) essential oil versus non-selective sandalwood (Tan Xiang) essential oil on cultured bladder cancer cells: a microarray and bioinformatics study. Chinese Medicine, 9, 18.

Publication 2014

fetal bovine serum MEM vitamin solution non-essential amino acids epidermal growth factor sodium pyruvate penicillin-streptomycin XTT cell proliferation assay kit RNeasy® Mini Kit

Assay Cell Cell culture Cell proliferation Culture medium Epidermal growth factor Essential amino acids Essential oils Fetal bovine serum Frankincense Insulin Penicillin Pyruvate Sandalwood Sodium Sodium selenite Streptomycin Supplement Transferrin Vitamin

Corresponding Organization :

Other organizations : Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, Dhofar University, University of Oklahoma Medical Center

Top 5 similar protocols

Variable analysis

independent variables

Frankincense (B. carterii from Somalia) essential oil
Sandalwood (S. album from Sri Lanka) essential oil

dependent variables

Cell proliferation (measured by XTT assay)

control variables

Cell culture medium (MEM and DMEM/F-12 (1:1))
Fetal bovine serum (FBS)
MEM vitamin solution
Non-essential amino acids
Epidermal growth factor (EGF)
Insulin-transferrin-sodium selenite (ITS) media supplement
Sodium pyruvate
Penicillin-streptomycin

Annotations

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