All libraries were independently prepared from separately cultured samples in duplicate, according to previously described protocols51 (link)–53 (link). Size selection was performed by extracting 200–400 bp DNA fragments on a 2% agarose E-gel (Invitrogen) before PCR amplification, then extracted using RNeasy MinElute cleanup kits (Qiagen). PCR amplification was performed using ≤ 14 cycles, and the resulting DNA quantified by Qubit fluorometric quantitation. Sequencing was performed using single-end reads on the Illumina HiSeq2000 sequencer.
Protocol detail
DNA Library Preparation and Sequencing
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Howard Hughes Medical Institute
Other organizations : Stanford University, National Heart Lung and Blood Institute, National Institutes of Health, Dana-Farber Cancer Institute, Harvard University
Top 5 similar protocols
Variable analysis
independent variables
- Separately cultured samples
- Sequencing data
- Previously described protocols
- Size selection (200-400 bp DNA fragments)
- PCR amplification (≤ 14 cycles)
- Qubit fluorometric quantitation
- Illumina HiSeq2000 sequencer
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!