Bone Marrow-Derived Macrophage Isolation

Bone marrow-derived macrophages (BMDMs) were primary cultured as previously described [55 (link)]. BMDMs were harvested from adult 6–8-week-old C57BL/6 mice with 7 days of culture in the cell culture medium, which was Dulbecco’s Modified Eagle Medium (DMEM) (Corning, NY, USA) supplemented with 5% newborn calf serum (NCS) (Rocky Mountain Biologicals, Missoula, MT, USA) and 15% conditional medium from the macrophage-colony stimulating factor secreting L929 fibroblast cell line (ATCC, Manassas, VA, USA).

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Cheng Z., Zhu W., Cao K., Wu F., Li J., Wang G., Li H., Lu M., Ren Y, & He X. (2016). Anti-Inflammatory Mechanism of Neural Stem Cell Transplantation in Spinal Cord Injury. International Journal of Molecular Sciences, 17(9), 1380.

Publication 2016

DMEM L929 fibroblast cell line

Adult Biologicals Bone marrow derived macrophages C57bl mice Cell Cell culture Eagle Fibroblast L929 cell line Macrophage colony stimulating factor Newborn Serum

Corresponding Organization :

Other organizations : Second Affiliated Hospital of Xi'an Jiaotong University, Xian Yang Central Hospital, Hunan Normal University, Florida State University

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Variable analysis

independent variables

Culture duration (7 days)

dependent variables

Bone marrow-derived macrophage (BMDM) characteristics

control variables

Mouse strain (C57BL/6)
Cell culture medium (Dulbecco's Modified Eagle Medium (DMEM) supplemented with 5% newborn calf serum (NCS) and 15% conditional medium from the macrophage-colony stimulating factor secreting L929 fibroblast cell line)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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