Lipid Extraction and Characterization Protocol

The phospholipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-hexadecanoyl-2-(9-Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (POPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine (POPS), and Liss-Rhod-DOPE were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cholesterol was obtained from Sigma Aldrich (Shanghai, China). Sodium chloride, chloroform, 4-hydroxyethyl piperazine sulfonic acid (HEPES), hydrochloric acid, sodium hydroxide and propranolol hydrochloride were of analytical grade and were purchased from Titan (Shanghai, China). Microscope coverslips (22 × 40 mm, no. 1.5) was supplied by Fisher Scientific (Pittsburgh, Pennsylvania, USA). PDMS (Dow Corning Sylgard Silicone Elastomer-184) was provided from Krayden, Inc. (El Paso, TX). Purified water (18.25 mΩ cm) was produced from a Direct-pure UP Water System (RephiLe Bioscience, Ltd, China).

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He N, & Zhao T. (2023). Propranolol induces large-scale remodeling of lipid bilayers: tubules, patches, and holes. RSC Advances, 13(11), 7719-7730.

Publication 2023

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine 1-hexadecanoyl-2-(9-Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine Liss-Rhod-DOPE

1 palmitoyl 2 oleoyl sn glycero 3 phosphoserine Alabaster Chloroform Cholesterol Glycerol Hydrochloric acid Lipids Microscope Phosphoethanolamine Phospholipids Piperazine Propranolol hydrochloride Silicone elastomer Sn glycero 3 phosphocholine Sodium chloride Sodium hydroxide Sulfonic acid

Corresponding Organization : Shanghai University of Engineering Science

Top 5 similar protocols

Variable analysis

independent variables

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)
1-hexadecanoyl-2-(9-Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (POPE)
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG)
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine (POPS)
Liss-Rhod-DOPE
Cholesterol
Sodium chloride
Hydrochloric acid
Sodium hydroxide
Propranolol hydrochloride

dependent variables

Not explicitly mentioned

control variables

Microscope coverslips (22 × 40 mm, no. 1.5)
PDMS (Dow Corning Sylgard Silicone Elastomer-184)
Purified water (18.25 mΩ cm)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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