To detect PCV2, 10% homogenates were prepared from each tissue sample, 50 µL of which was incubated with 2 µL of proteinase K at 37°C for 2 hr. Subsequently, proteinase K was inactivated by
heating the cell suspensions at 95°C for 2 min. The suspensions were centrifuged at 15,000 rpm for 5 min at room temperature, and the collected supernatant was used as the source of
extracted DNA. qPCR was performed as previously described [26 (link)].
To detect PRRSV, RNA extraction was performed as previously described [40 (link)]. The isolated RNA was then processed using a commercial RT-PCR kit (LSI
VetMAX PRRSV EU/NA, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
heating the cell suspensions at 95°C for 2 min. The suspensions were centrifuged at 15,000 rpm for 5 min at room temperature, and the collected supernatant was used as the source of
extracted DNA. qPCR was performed as previously described [26 (link)].
To detect PRRSV, RNA extraction was performed as previously described [40 (link)]. The isolated RNA was then processed using a commercial RT-PCR kit (LSI
VetMAX PRRSV EU/NA, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
