Quantitative gene expression analysis

Total RNA was isolated with over 1000 young adult worms using TransZol Up (Code#ET111–01, TransGen Biotech, Beijing, China), and reversed to cDNA using Prime Script RT reagent Kit (Cat#RR047A, Takara Bio Inc. Kusatsu, Japan). SYBR1 × green mix (Trans Start TipTop Green qPCR SuperMix, Cat#AQ141, TransGen Biotech, Beijing, China) was used for the qPCR reaction. The real-time amplification was monitored by ABI 7900HT analyzer (Applied Biosystems, Foster City, CA, USA). Relative expression of genes was calculated according to the ΔΔCt method [42 (link)]. β-Actin was used as the reference gene.

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Xiao R.Y., Hao J., Ding Y.H., Che Y.Y., Zou X.J, & Liang B. (2016). Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans. Molecules, 21(10), 1379.

Publication 2016

TransZol Up Prime Script RT reagent Kit Trans Start TipTop Green qPCR SuperMix ABI 7900HT analyzer

Actin Cdna Expression genes Gene Worms Young adult

Corresponding Organization : Kunming University

Other organizations : Heilongjiang University of Chinese Medicine, Yunnan University of Traditional Chinese Medicine

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Relative expression of genes

control variables

Total RNA was isolated with over 1000 young adult worms
β-Actin was used as the reference gene

controls

Positive control: None mentioned
Negative control: None mentioned

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