Whole-cell extracts of Synechococcus 7002 were prepared as described previously [75 (link)] and proteins were separated by SDS-PAGE [76 (link)] or BN-PAGE using precast NativePAGE Novex 4–16% Bis–Tris gels (Thermofisher, Germany). Preparation of thylakoid membranes for BN-PAGE analysis (Additional file 10) and gel electrophoresis was performed according to Lassen et al. [77 (link)].
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