Protocol detail

Chromatin Immunoprecipitation and Re-ChIP

Find Similar Protocols

ChIP and Re-ChIP assays were performed essentially as described before (29 (link)). Aliquots of lysates containing 200 μg of protein were used for each immunoprecipitation reaction with anti-MRTF-A (Santa Cruz, SC-32909), anti-BRG1 (Santa Cruz, SC-10768), anti-BRM (Santa Cruz, SC-6450), anti-acetyl H3 (Millipore, 06–599), anti-acetyl H4 (Millipore, 06-598), anti-dimethyl (Millipore, 07-030), anti-trimethyl H3K4 (Millipore, 07-473), anti-ASH2 (Bethyl Laboratories, A300-489A) or pre-immune IgG. Precipitated genomic DNA was amplified by real-time PCR with primers listed in Supplementary Table S1.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Yang Y., Cheng X., Tian W., Zhou B., Wu X., Xu H., Fang F., Fang M, & Xu Y. (2014). MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells. Nucleic Acids Research, 42(16), 10460-10472.

Publication 2014

anti-MRTF-A anti-BRG1 anti-acetyl H3 anti-acetyl H4 anti-trimethyl H3K4 anti-ASH2

Brg1 Chip Chip assays Genomic Immunoprecipitation Primers Protein Real time pcr

Top 5 similar protocols

Variable analysis

independent variables

Anti-MRTF-A (Santa Cruz, SC-32909)
Anti-BRG1 (Santa Cruz, SC-10768)
Anti-BRM (Santa Cruz, SC-6450)
Anti-acetyl H3 (Millipore, 06–599)
Anti-acetyl H4 (Millipore, 06-598)
Anti-dimethyl (Millipore, 07-030)
Anti-trimethyl H3K4 (Millipore, 07-473)
Anti-ASH2 (Bethyl Laboratories, A300-489A)

dependent variables

Precipitated genomic DNA

control variables

Aliquots of lysates containing 200 μg of protein
Pre-immune IgG

controls

Pre-immune IgG (negative control)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!