Paraffin-embedded KS specimens from HIV-negative and high-viral-load HIV-positive patients were collected at MacKay Memorial Hospital. The ethics of the study was approved by the Institutional Review Board of National Yang-Ming University (YM107135E) and MacKay Memorial Hospital (18MMHIS050e). Tissue sections were stained by immunohistochemistry (IHC) as described in our previous report (56 (link)). Anti-HIV Tat antibody (Abcam; ab43014) was used at a 1:100 dilution. The histology was evaluated by Tung-Ying Chen and validated by a board-certified pathologist, Chin-Chen Pan, in a blind fashion.
Yang W.S., Lin T.Y., Chang L., Yeh W.W., Huang S.C., Chen T.Y., Hsieh Y.T., Chen S.T., Li W.C., Pan C.C., Campbell M., Yen C.H., Chen Y.M, & Chang P.C. (2020). HIV-1 Tat Interacts with a Kaposi’s Sarcoma-Associated Herpesvirus Reactivation-Upregulated Antiangiogenic Long Noncoding RNA, LINC00313, and Antagonizes Its Function. Journal of Virology, 94(3), e01280-19.
Anti hiv antibody BlindChin Dilution Embedded paraffin Hiv positiveImmunohistochemistry Institutional review board PathologistPatients Tissue Tung
Corresponding Organization :
Other organizations :
National Yang Ming Chiao Tung University, Mackay Medical College, Mackay Memorial Hospital, National Yang Ming University Hospital, National Taipei University, Taipei Veterans General Hospital, University of California, Davis, Kaohsiung Medical University, Taipei Medical University
HIV status (HIV-negative and high-viral-load HIV-positive)
dependent variables
Expression of HIV Tat protein (measured by anti-HIV Tat antibody staining)
control variables
Paraffin-embedded KS specimens
Tissue sections stained by immunohistochemistry (IHC) as described in the previous report
positive controls
Not explicitly mentioned
negative controls
Not explicitly mentioned
Annotations
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