DNA Isolation and Genotyping Protocol

DNA was isolated using DNEasy Blood and Tissue Kit (QIAGEN, Valencia, CA) according to manufacturer’s instructions. RNO17 SNP genotyping (S1 Table) was performed using custom TaqMan^® genotyping assays (Life Technologies), according to manufacturer’s instructions on StepOnePlus^® thermal cyclers (Applied Biosystems). For genome-wide genotyping during the generation of the consomic strain, a custom GoldenGate genotyping BeadChip array (Illumina, San Diego, CA) was used as previously described [19 (link)]. The array contains 1,536 SNPs, of which 453 SNPs are polymorphic between LH and LN at a resolution sufficient to determine the background genome was fixed for the LH allele. Genotyping was performed at GeneSeek (Neogen Corporation, Lincoln, NE). SNP calls with GenCall scores exceeding 0.4 were accepted for analysis.