Western Blot Protein Analysis Protocol

Western blots was performed as described before.^{47 (link)} Western blots images were acquired using the LAS-4000 CCD imaging system (Fujifilm, Japan). The following antibodies were used: Rabbit polyclonal antibodies against KLF5 (Proteintech Group, Rosemont, IL, USA, 21017-1-AP), VHL (Novus Biologicals, Littleton, CO, USA, NB100-485), DNMT1 (Cell Signaling Technology, Beverly, MA, USA, #5032), DNMT3A (Cell Signaling Technology, #D23G1), Mouse monoclonal antibody against DNMT3B (Santa Cruz Biotechnology, sc-376043), HRP-linked β-actin monoclonal antibody (Proteintech Group, HRP-60008) and HRP-linked α-tubulin polyclonal antibody (MBL International Corporation, MA, USA, PM0547). The protein levels were quantified by Quantity One software.