As an initial screening for NVAV infection, total RNA was extracted from 20–50 mg of mole lung tissues, using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA), and cDNA was synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen), then analyzed for NVAV RNA by RT-PCR, using NVAV-specific oligonucleotide primers33 (link)34 (link).
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