Western Blot Analysis for Lipid Metabolism

Cell treatment procedures were performed as described for the qRT-PCR analyses. The same method was used as the one employed in our previous study (Zhang et al., 2017 (link)) to collect samples and then to perform Western blot. Briefly, RIPA buffer containing 0.1% protease inhibitor (Amerso, USA) was used to homogenize collected cell samples. The protein concentrations in the supernatants were measured by using BCA Protein Assay Kits (CW0014, CwBio, Inc., Beijing, China). The following antibodies were used: rabbit anti-HDAC2 (1:1,000), anti-HDAC3 (1:1,000), anti-LPL (1:1,000), anti-PPARγ (1:1,000) or anti-β-actin (1:1,000) antibody. β-actin was used as a reference standard to normalize protein levels. The results for Western blot were expressed as folds of control.