Visualizing Cells Using Spinning Disk Confocal Microscopy

Cells were incubated at 30°C in synthetic medium and grown to mid log phase. Cells were visualized using an Olympus IX71 microscope (Olympus) equipped with a CSU10 spinning-disk confocal scanner (Yokogawa Electric Corporation), as described previously (Yorimitsu and Sato, 2012 (link)). Images were captured using an electron-multiplying charge-coupled device camera (iXon, DV897; Andor Technology). Photoshop (Adobe) and ImageJ (National Institutes of Health) were used to prepare the images for figure preparation.

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Yorimitsu T, & Sato K. (2023). Sec16 and Sed4 interdependently function as interaction and localization partners at ER exit sites. Journal of Cell Science, 136(9), jcs261094.

Publication 2023

CSU10 spinning-disk confocal scanner

Cells Device Electric Electron Microscope

Corresponding Organization :

Other organizations : Tokyo University of the Arts, The University of Tokyo

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Variable analysis

independent variables

Incubation temperature (30°C)

dependent variables

Cell growth (to mid log phase)
Cell visualization (using Olympus IX71 microscope with CSU10 spinning-disk confocal scanner)

control variables

Synthetic medium
Microscope and camera setup (as described in Yorimitsu and Sato, 2012)

controls

No explicit positive or negative controls are mentioned in the provided information.

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