Isolation and Expansion of Primary BMSCs

BM samples were collected from MM patients for diagnosis and research purpose and blood was collected from healthy individuals after written informed consent in accordance with the Declaration of Helsinki. The research is approved by the Ethic Board of UZ Brussel (B.U.N. 143201316382) and the Tumourbank of Lille (CSTMT102). Mononuclear cells were obtained after Ficoll density gradient centrifugation (Nycomed, Lucron Bioproducts, Zurich, Switzerland) and purified as previously described [57 (link)]. Primary BMSCs were obtained from healthy subjects, and maintained in DMEM medium (Lonza, Basel, Switzerland) supplemented with 10% fetal calf serum (HyClone, USA), 10% horse serum (Invitrogen, USA), 100 U/mL penicillin/streptomycin (Lonza), and 2 mM L-glutamine (Lonza).