Trichoderma Strain Diversity and Cultivation

Eight Trichoderma strains, representing different genotypes, were included in this study: T. parareesei T6 (air, UK) [55 (link),56 (link)], T. virens T49 (soil, Brazil), T. longibrachiatum T68 (soil, Brazil), T. spirale T75 (solarized soil, Spain), T. koningii T77 (soil, Spain) [6 (link),55 (link)], and T. harzianum T115 (soil, Philippines) [6 (link)], T. hamatum T123 (Marchantia polymorpha rhizoids, Spain), and T. asperellum T140 (strawberry nursery soil, Spain) (references of our collection, CIALE, University of Salamanca, Spain). Strains were routinely grown on potato dextrose agar (PDA, Difco Laboratories, Detroit, MI, USA) at 28 °C in the dark. For long-term storage, the strains were maintained at −80 °C in a 30% glycerol solution. Conidia from 7-day-old PDA plates were harvested, and the conidia concentrations were calculated as previously described [35 (link)].