Fecal Microbiome Analysis in Mice

Stool samples were collected from each mouse in the 8^th week and immediately snap frozen in liquid nitrogen before storage at −80°C. The feces were collected after administration and extracted using a fecal DNA isolation kit (Qiagen, Germantown, USA). The extracted DNA from each sample was used as the template to amplify the V4 region of the 16S rRNA genes for subsequent pyrosequencing, which was performed by Novogene (Beijing, China) as described previously.^{47 (link)}

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Shi Y., Zou Y., Xiong Y., Zhang S., Song M., An X., Liu C., Zhang W, & Chen S. (2021). Host Gasdermin D restrains systemic endotoxemia by capturing Proteobacteria in the colon of high-fat diet-feeding mice. Gut Microbes, 13(1), 1946369.

Publication 2021

fecal DNA isolation kit

Fecal Frozen Isolation Mouse Nitrogen Rrna genes

Corresponding Organization : China Pharmaceutical University

Other organizations : Nanjing University of Chinese Medicine

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Variable analysis

independent variables

Time point (8th week)

dependent variables

Microbial composition based on 16S rRNA gene sequencing

control variables

Mouse samples
Fecal DNA extraction method (Qiagen fecal DNA isolation kit)
16S rRNA gene amplification and sequencing method (V4 region)

controls

No positive or negative controls were explicitly mentioned.

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