Protocol detail

Western Blot Analysis of Protein Markers

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Proteins extracted from the frozen specimens were homogenised in protein lysis buffer, loaded on SDS-PAGE and immunoblotted as previously described.^{10 (link)} An Odissey FC Imaging System (LI-COR) was used for acquisition. Primary anti-CCAAT Enhancer Binding Protein Alpha (C/EBPα)(1:150, cat.#sc-9314) and actin (1:500, cat.#sc-1616) were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Primary anti-UVSSA (1:500, cat.# A09174) was purchased from Biocompare- BosterBio (Pleasanton, California, USA).

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Bello E., Brich S., Craparotta I., Mannarino L., Ballabio S., Gatta R., Marchini S., Carrassa L., Matteo C., Sanfilippo R., Gronchi A., Casali P.G., Pilotti S., D’Incalci M, & Frapolli R. (2019). Establishment and characterisation of a new patient-derived model of myxoid liposarcoma with acquired resistance to trabectedin. British Journal of Cancer, 121(6), 464-473.

Publication 2019

Odissey FC Imaging System actin

Actin Buffer Ccaat enhancer binding protein alpha Frozen Protein Sds page

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Variable analysis

independent variables

Protein extraction from frozen specimens

dependent variables

Protein expression levels of CCAAT Enhancer Binding Protein Alpha (C/EBPα)
Protein expression levels of UVSSA

control variables

Protein lysis buffer
SDS-PAGE
Immunoblotting procedure
Actin as a loading control

controls

Positive control: Actin (used as a loading control)
Negative control: Not explicitly mentioned

Annotations

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