The concentration of chlorophyll (Chl) of one gram of fresh leaves was extracted with 5 mL N,N-dimethyl-formamid overnight at 5 °C, then Chl. a and b were estimated using a spectrophotometer at 663 and 647 nm as mg/g f w [67 (link)]. The concentrations were calculated with the following equations:
Free full text: Click here