As mentioned previously,7 (link)17 (link) a stable mouse model was established using 70% partial hepatic heat IR. Mice were anesthetized and a laparotomy was performed to expose the liver. The left and middle branches of the intrahepatic portal vein were clamped to block the blood supply. The clips were released for reperfusion after 90 minutes of ischemia. The sham mice underwent the same procedure but without clamping the blood vessels. The mice were sacrificed 6 hours after reperfusion, and liver and blood samples were collected for analysis.
To study the effects of ER stress, Control and HFD-fed mice were injected with tauroursodeoxycholic acid (TUDCA) i.p. (400 mg/kg) or PBS (Control) for 3 days, and then the model of liver IR was established.
To determine the role of mitochondrial oxidation, Control and HFD-fed mice were pretreated with the mitochondria-targeted antioxidant Mito-TEMPO (5 mg/kg, MedChemExpress, HY-112879) twice at 17 hours and 1 hour before surgery or PBS.
To study the effects of ER stress, Control and HFD-fed mice were injected with tauroursodeoxycholic acid (TUDCA) i.p. (400 mg/kg) or PBS (Control) for 3 days, and then the model of liver IR was established.
To determine the role of mitochondrial oxidation, Control and HFD-fed mice were pretreated with the mitochondria-targeted antioxidant Mito-TEMPO (5 mg/kg, MedChemExpress, HY-112879) twice at 17 hours and 1 hour before surgery or PBS.
