Hippocampal Cytoarchitecture Evaluation

The evaluations focused on the hippocampus. To analyse the gross morphology and neuronal cytoarchitecture of dentate gyrus (DG) and Cornus Ammonis (CA) hippocampal areas by light microscopy, H&E staining was performed as previously reported [39 (link),42 (link)]. Briefly, approximately 20 randomised sections (5 microscopic fields) per animal and time/condition were examined by a blinded operator using a Leica DM6B WF microscope (Leica Microsystems, Buccinasco, MI, Italy). The images were acquired with Leica dfc 7000 t CCD camera (Leica microsystems, Buccinasco, MI, Italy) and stored on a PC running the Leica Application Suite X (LAS X) software (Version 5.1.0).

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Priori E.C., Ratto D., De Luca F., Sandionigi A., Savino E., Giammello F., Romeo M., Brandalise F., Roda E, & Rossi P. (2023). Hericium erinaceus Extract Exerts Beneficial Effects on Gut–Neuroinflammaging–Cognitive Axis in Elderly Mice. Biology, 13(1), 18.

Publication 2023

Leica dfc 7000 t CCD camera

Corresponding Organization : Istituti Clinici Scientifici Maugeri

Other organizations : University of Milano-Bicocca, University of Milan

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Variable analysis

independent variables

Time/condition

dependent variables

Gross morphology and neuronal cytoarchitecture of dentate gyrus (DG) and Cornus Ammonis (CA) hippocampal areas

control variables

Number of randomised sections (approximately 20 per animal and time/condition)
Number of microscopic fields (5 per animal and time/condition)
Blinded operator
Leica DM6B WF microscope
Leica dfc 7000 t CCD camera
Leica Application Suite X (LAS X) software (Version 5.1.0)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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