Protein Expression Analysis by Western Blot

The total proteins were collected by lysis buffer with protease inhibitors. The detection procedure of specific proteins levels by western blot was listed in our previous study [16 (link)]. The primary antibodies (1:1000) were shown as follows: rabbit anti-Bcl2 antibody (Boster, China), rabbit anti-DOCK1 antibody (CST, USA), rabbit anti-E-cadherin antibody (Proteintech, USA), rabbit anti-Ezrin antibody (Proteintech, USA), mouse anti-GAPDH antibody (CST, USA), rabbit anti-MMP9 antibody (CST, USA), rabbit anti-tubulin antibody (ABclonal, China), rabbit anti-phospho-C-Raf (S338) antibody (CST, USA), rabbit anti-C-Raf antibody (CST, USA), rabbit anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (CST, USA), rabbit anti-ERK1/2 antibody (CST, USA). The chemiluminescence method was used to display the bands.