Induction of Fbxo9 cKO and Cbfb-MYH11 Expression

Fbxo9 cKO mice were developed using Easi-CRISPR, as previously published [29 (link)], and bred with Cbfb^+/56M [29 (link),32 (link)] or Mx-cre mice, purchased from Jackson Laboratories (#003556, Bar Harbor, ME, USA). PCR confirmed the genotype and the expression of these genes (primers listed in Supplemental Materials and Methods). To induce cKO of Fbxo9 and expression of Cbfb-MYH11, 6–8 week-old floxed mice and littermate controls received three intraperitoneal Poly(I:C) injections every other day, at a dose of 10 µg per gram body weight (Invivogen, San Diego, CA, USA). Procedures performed were approved by the Institutional Animal Care and Use Committee of the University of Nebraska Medical Center in accordance with NIH guidelines (protocol number: 15-099-11-FC).