Tea Plant CsCAMTAs Expression Analysis

RNA extraction kit (Bioflux, Hangzhou, China) and RT reagent Kit (Takara, Dalian, China) were respectively used to isolate total RNA and synthesize the first-strand cDNA following the corresponding instruction of kits. The qRT-PCR technique was performed as described by Wang et al. (2021) [35 (link)]. In brief, total of 20.0 μL reaction mix (10.0 μL SYBR Premix Ex Taq, 2 μL cDNA, 1.6 μL forward/reverse primers, and 6.4 μL distilled water) were amplified according to the following qRT-PCR programs: 95 °C, 15 s (step 1); 94 °C, 5 s following 60 °C, 30 s with 40 cycles (step 2); adding melting curve (step 3). A reference gene, polypyrimidine tract-binding protein (CsPTB) of tea plant [57 (link)] was used to quantify the relative expression levels of CsCAMTAs. The results were calculated by 2^−ΔCt or 2^−ΔΔCt method [58 (link)], and finally visualized as the mean values ± standard error (± SE). The qRT-PCR primers are listed in Table S2.