Viability Assays for hBMECs

Live/dead and CyQuant viability assays were performed as previously described (12 (link)). For live/dead assays, hBMECs were costained with calcein-AM (live/green, 3 μM; Invitrogen) and propidium iodide (dead/red, 2.5 μM; Calbiochem). calcein-AM-positive versus PI-positive cells were resolved using an Olympus IX51 microscope and overlaid using Adobe Photoshop. hBMECs were incubated with CyQuant-NF (Thermo Fisher), and fluorescence was quantified using a BioTek FLx800 fluorimeter.

Free full text: Click here

Mladinich M.C., Conde J.N., Schutt W.R., Sohn S.Y, & Mackow E.R. (2021). Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells. mBio, 12(4), e01962-21.

Publication 2021

calcein-AM IX51 microscope CyQuant-NF FLx800 fluorimeter

Assays Calcein Cells Fluorescence Microscope Propidium iodide

Corresponding Organization : Stony Brook University

Top 5 similar protocols

Variable analysis

independent variables

Incubation of hBMECs with different assays (Live/dead and CyQuant viability assays)

dependent variables

Live versus dead cells (resolved using Olympus IX51 microscope and overlaid using Adobe Photoshop)
Fluorescence quantification of hBMECs incubated with CyQuant-NF using a BioTek FLx800 fluorimeter

control variables

Calcein-AM (live/green, 3 μM; Invitrogen)
Propidium iodide (dead/red, 2.5 μM; Calbiochem)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!