Briefly, the transfected H1299 and H358 cells were seeded into 60-mm dishes (Corning, NY, USA) in triplicate at a density of 2000 cells/well, followed by incubation at 37 °C for 14 days. The colonies were fixed with 4% paraformaldehyde for 15 min, stained with crystal violet at room temperature for 30 min, and then counted [30 (link)].
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