Colony Formation Assay for Cell Lines
Corresponding Organization : Xinqiao Hospital
Other organizations : Zhejiang University
Variable analysis
- Transfection of H1299 and H358 cells
- Colony formation
- Seeding density of 2000 cells/well
- Incubation at 37 °C for 14 days
- Fixation with 4% paraformaldehyde for 15 min
- Staining with crystal violet at room temperature for 30 min
- No positive or negative controls were explicitly mentioned in the provided information.
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