Immunofluorescence was performed as described [47 (link)] using antibodies against Podocalyxin (catalog # PA1-46169; Thermo Fisher Scientific) and Syntaxin-4 (catalog # 610439; BD Biosciences; San Jose, CA). Confocal analyses were performed using an Olympus FV3000 confocal microscopy system (Olympus; Waltham, MA).
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Podocalyxin and Syntaxin-4 Visualization
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Corresponding Organization :
Other organizations : University of California, San Diego, City of Hope
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- Antibodies used: Podocalyxin (catalog # PA1-46169; Thermo Fisher Scientific) and Syntaxin-4 (catalog # 610439; BD Biosciences; San Jose, CA)
- Immunofluorescence analysis
- Confocal microscopy analysis
- Immunofluorescence was performed as described [47 (link)]
- Confocal analyses were performed using an Olympus FV3000 confocal microscopy system (Olympus; Waltham, MA)
- No positive or negative controls were explicitly mentioned.
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