Hepatic Injury Assessment Protocol

Plasma levels of aminotransferases (alanine aminotransferase [ALT] and aspartate aminotransferase [AST]) and hepatic levels of triglycerides were determined using standard kits (Thermotrace, Melbourne, Australia).4 (link) Paraffin-embedded sections were stained for hematoxylin and eosin (H&E); pathology was scored in a blinded manner by a trained pathologist.8 (link) Oil Red O staining,4 (link) chloroacetate esterase (CAE) staining, and neutrophil accumulation in the livers were assessed as described.8 (link),9 (link) The intensity and extent of CAE staining in liver tissues were quantified by counting CAE-positive neutrophils per 1000 hepatocytes. Plasma thrombin-antithrombin (TAT) concentration was determined by enzyme-linked immunosorbent assay using a kit (Dade Behring Inc., Deerfield, IL).10 (link) The accumulation of fibrin matrices was determined immunofluorometrically as described.8 (link)

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Beier J.I., Luyendyk J.P., Guo L., von Montfort C., Staunton D.E, & Arteel G.E. (2009). Fibrin Accumulation Plays a Critical Role in the Sensitization to Lipopolysaccharide-Induced Liver Injury Caused by Ethanol in Mice. Hepatology (Baltimore, Md.), 49(5), 1545-1553.

Publication 2009

Alanine aminotransferase Aminotransferases Antithrombin Aspartate aminotransferase Chloroacetate esterase Dade Enzyme linked immunosorbent assay Eosin Fibrin Hepatocytes Il 10 Liver Neutrophils Paraffin Pathologist Plasma Thrombin Tissues Triglycerides

Corresponding Organization : GTx (United States)

Other organizations : University of Kansas Medical Center, CisThera (United States)

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Protocol cited in 6 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma levels of aminotransferases (alanine aminotransferase [ALT] and aspartate aminotransferase [AST])
Hepatic levels of triglycerides
Pathology score (determined by a trained pathologist)
Intensity and extent of chloroacetate esterase (CAE) staining in liver tissues (quantified by counting CAE-positive neutrophils per 1000 hepatocytes)
Plasma thrombin-antithrombin (TAT) concentration
Accumulation of fibrin matrices (determined immunofluorometrically)

control variables

None explicitly mentioned

positive controls

None specified

negative controls

None specified

Annotations

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