Antibody Validation for Protein Signaling

Mouse anti-Myc (9E10), rabbit anti-HA (F-7), mouse anti-α-tubulin (B-7) and mouse anti-β-tubulin (3F3-G2) antibodies were purchased from Santa Cruz. Mouse anti-Flag (F3165), rabbit anti-Flag (F7425) and mouse anti-ubiquitin (P4D1) antibodies were purchased from Sigma-Aldrich. Rabbit anti-cGAS (D1D3G), rabbit anti-STING (D2P2F) and rabbit anti-TBK1 (D1B4) antibodies were from Cell Signaling Technology. Mouse anti-V5 (E10/V4RR) antibodies were from Invitrogen.

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Lui W.Y., Bharti A., Wong N.H., Jangra S., Botelho M.G., Yuen K.S, & Jin D.Y. (2023). Suppression of cGAS- and RIG-I-mediated innate immune signaling by Epstein-Barr virus deubiquitinase BPLF1. PLOS Pathogens, 19(2), e1011186.

Publication 2023

Mouse anti-Flag (F3165), rabbit anti-Flag (F7425)

Antibodies Cgas Mouse Rabbit Tbk1 Tubulin Ubiquitin α tubulin

Corresponding Organization : Tung Wah College

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Variable analysis

independent variables

Mouse anti-Myc (9E10)
Rabbit anti-HA (F-7)
Mouse anti-α-tubulin (B-7)
Mouse anti-β-tubulin (3F3-G2)
Mouse anti-Flag (F3165)
Rabbit anti-Flag (F7425)
Mouse anti-ubiquitin (P4D1)
Rabbit anti-cGAS (D1D3G)
Rabbit anti-STING (D2P2F)
Rabbit anti-TBK1 (D1B4)
Mouse anti-V5 (E10/V4RR)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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