Human androgen-refractory PCa ARCaPE and ARCaPM and LNCaP, LNCaPNeo and LNCaPRANKL PCa (15 (link)–17 (link)) were used. PCa cells and 293T cells were cultured in T-medium (GibcoBRL) supplemented with 5% heat inactivated fetal bovine serum (Bio-Whittaker) as previously mentioned (18 (link)). All cells were tested for mycoplasma every three months and were negative. The embryonic stem cells and iPSCs derived small RNA preparations were provided by Drs. Sareen and Svendsen. Derivation of these cells is included in the Supplementary materials and methods and figure legends.
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Evaluating Human Androgen-Refractory Prostate Cancer Cell Lines
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293t cells
Androgen
Cells
Cultured medium
Embryonic stem cells
Fetal bovine serum
Human
Ipscs
Mycoplasma
Corresponding Organization :
Other organizations : Samueli Institute, Life Systems (United States), University of British Columbia, Cedars-Sinai Medical Center
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Variable analysis
independent variables
- Human androgen-refractory PCa ARCaP_E and ARCaP_M and LNCaP, LNCaP^Neo and LNCaP^RANKL PCa cell lines
- Measured outcomes (not explicitly mentioned)
- All cells were tested for mycoplasma every three months and were negative
- PCa cells and 293T cells were cultured in T-medium (GibcoBRL) supplemented with 5% heat inactivated fetal bovine serum (Bio-Whittaker)
- Positive control: Embryonic stem cells and iPSCs derived small RNA preparations
- Negative control: Not explicitly mentioned
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