Lung Cancer Model Induction and Statin Treatment

All animal experiments were performed under UK Home Office Licence authority. Braf^{+/LSL−V600E}, Kras^+/LSL−G12D and CCAGCreER^TM mice were backcrossed onto the C57BL/6J strain and genotyped as reported (Kamata et al., 2017 (link), 2020 (link)). Male and female animals >8 weeks of age were used for experimental procedures. Nasal delivery of Ad5-CMV-Cre or Ad5-mSPC-Cre was performed as described (Kamata et al., 2017 (link), 2020 (link)). Atorvastatin (10 mg/kg, Generon) was orally administered once daily for the indicated periods. Lung tissues were processed for Haematoxylin and Eosin (H&E) staining and immunohistochemistry (IHC) as described (Kamata et al., 2020 (link)).

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Kamata T., Al Dujaily E., Alhamad S., So T.Y., Margaritaki O., Giblett S., Pringle J.H., Le Quesne J, & Pritchard C. (2022). Statins mediate anti- and pro-tumourigenic functions by remodelling the tumour microenvironment. Disease Models & Mechanisms, 15(2), dmm049148.

Publication 2022

Atorvastatin

Animals Atorvastatin Braf Delivery Eosin Female Haematoxylin Immunohistochemistry Kras Lung Male Mice Nasal Strain Tissues

Corresponding Organization :

Other organizations : Leicester Royal Infirmary, University of Leicester

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Variable analysis

independent variables

Nasal delivery of Ad5-CMV-Cre or Ad5-mSPC-Cre
Atorvastatin (10 mg/kg, Generon) oral administration

dependent variables

Lung tissue histology (Haematoxylin and Eosin (H&E) staining)
Lung tissue immunohistochemistry (IHC)

control variables

Male and female animals >8 weeks of age
C57BL/6J mouse strain

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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