The study was carried out with 72 Wistar Albino male rats, weighing between 200–300 g, taken from the Experimental Research and Application Center (ATADEM) laboratory of Atatürk University. The water intake and pellet intake of the animals were ad libitum. Before starting the experiment, the rats were kept in cages for 1 week at normal room temperature (22 °C) in order to adapt to the environment. The ethics committee approval of the study was approved by the Animal Experiments Local Ethics Committee of Atatürk University with the document dated 11 January 2022 and numbered E-42190979-000-2200008858. A total of 72 male rats were randomized into 6 groups (5 experimental groups and 1 control group, 12 animals in each group). Before starting the experiment, all of the animals were weighed, and their weights were recorded. Imidacloprid (5.7 mg/kg), acetamiprid (12.4 mg/kg) and S. Boulardii (1 × 109 CFU/day) were given orally to the animals, divided into groups, for 90 days [5 (link),27 (link),28 (link)].
The animals were sacrificed 90 days after injection. The rats were decapitated rapidly under deep anesthesia (Sevoflurane). One testis of each animal in each group was collected and fixed in 10% neutral formaldehyde for immunohistochemical analysis (Sigma-Aldrich, St. Louis, MO, USA).
The animals were sacrificed 90 days after injection. The rats were decapitated rapidly under deep anesthesia (Sevoflurane). One testis of each animal in each group was collected and fixed in 10% neutral formaldehyde for immunohistochemical analysis (Sigma-Aldrich, St. Louis, MO, USA).
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