Dextran Sulfate Sodium-Induced Colitis in Mice

Mice 6- to 8-week-old were fed ad libitum for one cycle with water containing 2% (wt/vol) DSS (MW 40,000 kDa; MP Biomedicals Inc.) for 7 days followed by 8 days with regular water without DSS (68 (link)). Mice were monitored daily for changes in body weight, stool consistency and rectal bleeding. These values were used to calculate the Disease Activity Index (DAI). Endoscopy was performed using Coloview endoscopic system (Karl Storz, Germany) at day 8. Colonic inflammation was scored according to MEICS (Murine Endoscopic Index of Colitis Severity) as described (69 (link)). Mice were sacrificed at indicated time points or at the end of the protocol for the isolation of colonic tissue. Histological scoring was performed on hematoxylin and eosin (H&E) stained colon sections as previously described (70 (link)). Scores of inflammation and epithelial damage were summed to produce the overall histological score. All histological assessments were performed in a blinded fashion, by two independent investigators.

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Gargani S., Lourou N., Arapatzi C., Tzanos D., Saridaki M., Dushku E., Chatzimike M., Sidiropoulos N.D., Andreadou M., Ntafis V., Hatzis P., Kostourou V, & Kontoyiannis D.L. (2022). Inactivation of AUF1 in Myeloid Cells Protects From Allergic Airway and Tumor Infiltration and Impairs the Adenosine-Induced Polarization of Pro-Angiogenic Macrophages. Frontiers in Immunology, 13, 752215.

Publication 2022

DSS Coloview endoscopic system

Body weight Colitis Colon Endoscopic Eosin Inflammation Isolation Mice Stool Tissue

Corresponding Organization : Alexander Fleming Biomedical Sciences Research Center

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Variable analysis

independent variables

Dosage of DSS (2% wt/vol) in water

dependent variables

Body weight changes
Stool consistency
Rectal bleeding
Disease Activity Index (DAI)
Colonic inflammation score (MEICS)
Histological score (inflammation and epithelial damage)

control variables

Age of mice (6- to 8-weeks-old)
Feeding regime (ad libitum)
Duration of DSS exposure (7 days) and recovery period (8 days)

controls

Positive control: Mice fed water containing 2% (wt/vol) DSS for 7 days
Negative control: Mice fed regular water without DSS

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