Five lactobacilli strains (L. salivarius MG242 (KCTC18554P), L. fermentum MG901 (KFCC11651P), L. plantarum MG989 (KFCC11650P), L. paracasei MG4272 (KCTC13822BP), and L. rhamnosus MG4288 (KCTC13823BP)), isolated from a healthy Korean woman’s vagina, were supplied by Mediogen Co., Ltd. (Jecheon, Korea). All strains were deposited in the Korean Collection for Type Cultures (KCTC, Daejeon, Korea). Each strain was activated by culture in de Man, Rogosa, and Sharpe (MRS) broth (Difco, Detroit, MI, USA) for 18 h at 37 °C [20 (link)]. The dried bacterial powder used for in vivo testing was supplied by Mediogen Co., Ltd. The lactobacilli strain mixture (LM5) was prepared by combining the five powdered strains in equal ratios.
G. vaginalis (KCTC5096) was obtained from the KCTC and sub-cultured in modified brain heart infusion (mBHI) broth (Difco, Detroit, MI, USA) supplemented with yeast extract (1%), maltose (0.1%), glucose (0.1%), and horse serum (10%) and cultured anaerobically using Anaerocult® A (Merck, Darmstadt, Germany) in a sealed anaerobic jar.
G. vaginalis (KCTC5096) was obtained from the KCTC and sub-cultured in modified brain heart infusion (mBHI) broth (Difco, Detroit, MI, USA) supplemented with yeast extract (1%), maltose (0.1%), glucose (0.1%), and horse serum (10%) and cultured anaerobically using Anaerocult® A (Merck, Darmstadt, Germany) in a sealed anaerobic jar.
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