Immunohistochemical and immunofluorescent staining of allografts and colon biopsies were performed as previously described.21 (link) Cytokeratins, Ly6A/E and Dclk1 were stained using mouse anti-pancytokeratin monoclonal antibody (Novus Biologicals), rat anti-Ly6A/E (BD BioSciences) and rabbit polyclonal antibody to Dclk1 (Abcam). Rabbit anti-nitric oxide synthase 2 (Enzo Life Sciences), anti-arginase 1 (Sigma), and anti-MSH2 (Santa Cruz Biotechnology) polyclonal antibodies were used for Western blots.
Protocol detail
Immunostaining of Allografts and Biopsies
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Allografts
Anti antibody
Antibodies
Antibody
Arginase 1
Biologicals
Biopsies
Colon
Cytokeratins
Dclk1
Immunofluorescent
Monoclonal antibody
Mouse
Nitric oxide synthase 2
Novus
Rabbit
Western blots
Corresponding Organization :
Other organizations : Veterans Health Administration, University of Oklahoma Health Sciences Center
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Variable analysis
independent variables
- Immunohistochemical staining
- Immunofluorescent staining
- Expression of Cytokeratins
- Expression of Ly6A/E
- Expression of Dclk1
- Expression of nitric oxide synthase 2
- Expression of arginase 1
- Expression of MSH2
- Allografts
- Colon biopsies
- Positive control: Not specified
- Negative control: Not specified
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