VNTRs in DRD4 and SLC6A3 as well as the insertion/deletion in the promoter region of SLC6A4 genes were genotyped by PCR followed by electrophoretic analysis as previously described.25 (link),26 (link) DNA amplification reactions were performed with GoTaq® Green Master Mix (Promega), using the following cycling conditions: 94°C for 10 min, followed by 30 cycles of 94°C for 45 sec, 61°C for 45 sec, 72°C for 45 sec. Amplification was completed with a final elongation step of 72°C for 10 min. Fragments were separated on 1,2% agarose gels and visualized using ethidium bromide.
Polymorphisms in DBH (Taq1 A polymorphism) and HTR1B (rs6296) genes were genotyped by PCR-RFLP. For HTR1B the PCR product was digested with HincII restriction enzyme at 37°C overnight, and the PCR product of DBH was digested with Taq1 at 61°C for 16 hours. The alleles were detected after separation on an agarose gel. Experimental approaches were used as published before.27 (link),28 (link)The dinucleotide (CA)n repeat polymorphism located in the DRD5 gene was genotyped using PCR with fluorescently labeled primers, followed by capillary electrophoresis in an ABI 3500 sequencer.29 (link) Genotypes were analysed with Gene Mapper 4.1 software (Applied Biosystems).
Polymorphisms in DBH (Taq1 A polymorphism) and HTR1B (rs6296) genes were genotyped by PCR-RFLP. For HTR1B the PCR product was digested with HincII restriction enzyme at 37°C overnight, and the PCR product of DBH was digested with Taq1 at 61°C for 16 hours. The alleles were detected after separation on an agarose gel. Experimental approaches were used as published before.27 (link),28 (link)The dinucleotide (CA)n repeat polymorphism located in the DRD5 gene was genotyped using PCR with fluorescently labeled primers, followed by capillary electrophoresis in an ABI 3500 sequencer.29 (link) Genotypes were analysed with Gene Mapper 4.1 software (Applied Biosystems).
