Offgas analysis, biomass dry weight measurements, HPLC analysis of culture supernatants and correction for ethanol evaporation in bioreactor experiments were performed as previously described (Papapetridis et al.2016 (link)). Determination of optical density was performed at 660 nm using a Jenway 7200 spectrophotometer (Cole-Palmer, Staffordshire, UK). Yields of products and biomass-specific sugar uptake rates in bioreactor batch cultures were determined as previously described (Wisselink et al.2009 ; Papapetridis et al.2016 (link)). All values are represented as averages ± mean deviation of independent biological duplicate cultures.
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Bioreactor Cultivation Analysis Protocol
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Other organizations : Delft University of Technology
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Variable analysis
independent variables
- Offgas analysis
- Biomass dry weight measurements
- HPLC analysis of culture supernatants
- Correction for ethanol evaporation in bioreactor experiments
- Optical density at 660 nm
- Yields of products
- Biomass-specific sugar uptake rates in bioreactor batch cultures
- Independent variables not explicitly mentioned.
- Dependent variables not explicitly mentioned.
- Positive and negative controls not specified.
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