Prostate Cancer Cell Protein Analysis

Cell lysates were prepared from prostate cancer cells as previously described (26 (link)) and Western blot was performed using Mini-PROTEAN system (Bio-Rad). Primary antibodies against SREBP-1 (sc-8984), FASN (sc-48357), HMGCR (sc-33827), PSA (sc-7638) and β-actin (sc-47778) (Santa Cruz Biotechnology), SREBP-2 (ab72856, ab112046, Abcam), AR (PG-21, Millipore) and GAPDH (2118S, Cell Signaling Technology) and horseradish peroxidase-conjugated secondary antibodies (GE Healthcare Bio Science Corp) were used and membranes were visualized using a chemiluminescence reagent (Amersham Biosciences).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Li X., Chen Y.T., Hu P, & Huang W.C. (2014). Fatostatin Displays High Anti-Tumor Activity in Prostate Cancer by Blocking SREBP-Regulated Metabolic Pathways and Androgen Receptor Signaling. Molecular cancer therapeutics, 13(4), 855-866.

Publication 2014

Mini-PROTEAN system sc-8984 ab72856, ab112046 AR (PG-21, GAPDH horseradish peroxidase-conjugated secondary antibodies

Actin Antibodies Cells Chemiluminescence Fasn Gapdh Hmgcr Horseradish peroxidase Membranes Prostate cancer Srebp 1 Western blot

Corresponding Organization :

Other organizations : Air Force Medical University, Xijing Hospital, Cedars-Sinai Medical Center

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of SREBP-1, FASN, HMGCR, PSA, SREBP-2, AR, and GAPDH

control variables

Cell lysates from prostate cancer cells prepared as previously described (reference 26)
Western blot performed using Mini-PROTEAN system (Bio-Rad)
Primary antibodies against SREBP-1, FASN, HMGCR, PSA, β-actin, SREBP-2, AR, and GAPDH
Horseradish peroxidase-conjugated secondary antibodies (GE Healthcare Bio Science Corp)
Chemiluminescence reagent (Amersham Biosciences) used for visualization

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!