Sample analysis was performed using a system comprised of a UHPLC Vanquish pump, Vanquish Autosampler, Vanquish UHPLC+ column compartment, and ISQ EC mass spectrometer (ThermoFisherSci.), utilizing ThermoFisherScientific Dionex Chromeleon 7 Chromatography Data System software, as described [17 (link), 18 (link)]. A C18 column (4.6 mm ID X 100 mm L, 5 μm, 120 Å; Acclaim 120; ThermoFisherSci.) was used with a 0.25 mL/min. flow rate and 0.01–1 μL injection volume; the autosampler needle was washed with 10% (v/v) methanol (10 s) after each sample injection. Mobile phases A and B consisted of 10 mM ammonium acetate and acetonitrile, respectively. The isocratic mobile phase flow was characterized by a uniform concentration in acetonitrile 50% between 0 to 7 or 14 min.
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UHPLC-MS Analysis of Samples
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Corresponding Organization : University of Louisiana at Monroe
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Variable analysis
independent variables
- Flow rate (0.25 mL/min)
- Injection volume (0.01-1 μL)
- Sample analysis
- UHPLC Vanquish pump
- Vanquish Autosampler
- Vanquish UHPLC+ column compartment
- ISQ EC mass spectrometer (ThermoFisherSci.)
- ThermoFisherScientific Dionex Chromeleon 7 Chromatography Data System software
- C18 column (4.6 mm ID X 100 mm L, 5 μm, 120 Å; Acclaim 120; ThermoFisherSci.)
- Mobile phases A (10 mM ammonium acetate) and B (acetonitrile)
- Isocratic mobile phase flow with 50% acetonitrile concentration between 0 to 7 or 14 min
- Autosampler needle wash (10% (v/v) methanol, 10 s)
- No positive or negative controls were explicitly mentioned.
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