The mouse monoclonal anti-myc, anti-FLAG, anti-GFP, and anti-B-actin antibodies were obtained from Sigma (St. Louis, MO), while the anti-E-cadherin and anti-vimentin antibodies were purchased from BD Biosciences (San Diego, CA). The mouse polyclonal anti-TLE1 antibody was obtained from Abcam (Cambridge, MA) while the anti-Zeb1 antibody was purchased from Santa Cruz Biotechnology, Santa Cruz, CA). The FLAG-AES and the various plasmid constructs encoding for Bit1 were generated as described previously [9 (link)]. The GFP-TLE1 and the full length E-cadherin encoding plasmids were obtained from Origene (Rockville, MD).
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