Liver single cells were isolated by two-step collagenase digestion and gradient centrifugation. To capture CD133+ progenitor cells, the cells were labeled with primary CD133 antibody (mouse IgG1, Miltenyi Biotec) and subsequently magnetically isolated using the EasySep PE Selection Kit (Stemcell Technologies) according to the manufacturer’s instructions. A total of 5 × 105 CD133+ HPCs were injected into the spleen of each Fah−/− mouse as previously described (27 (link)).
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Isolation and Transplantation of CD133+ Liver Progenitor Cells
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Corresponding Organization : West China Hospital of Sichuan University
Other organizations : Weifang Medical University, Cincinnati Children's Hospital Medical Center, Rutgers, The State University of New Jersey
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Variable analysis
independent variables
- Isolation of CD133+ progenitor cells from liver single cells using primary CD133 antibody and magnetic isolation
- Engraftment and repopulation of CD133+ progenitor cells in Fah-/- mice
- Fah-/- mice
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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