Western blotting was carried as previously described.28 (link)-30 (link, link) Briefly, an equal amount of protein was separated by sodium dodecyl
sulfate polyacrylamide gel electrophoresis on a 10% gel electrophoresis and
transferred onto a 0.45-µm polyvinylidene fluoride membranes (PALL). The
membranes were blocked in 0.5% BSA at room temperature for 2 hours. The
following monoclonal primary antibodies were used in the Western blot analysis:
anti-NOCA2 (Abcam), anti-glyceraldehyde 3-phosphate dehydrogenase (CST),
anti-Slug (Abcam), anti-N-cad (Abcam), anti-E-cad (Abcam), anti-Vimentin
(Abcam), anti-β-catenin (Abcam), and anti-TCF-8 (Abcam), overnight at 4 °C. The
membranes were washed 3 times with TBST (0.1% Tween-20) for 10 minutes, each at
room temperature. The signal was visualized by chemiluminescence kit.
sulfate polyacrylamide gel electrophoresis on a 10% gel electrophoresis and
transferred onto a 0.45-µm polyvinylidene fluoride membranes (PALL). The
membranes were blocked in 0.5% BSA at room temperature for 2 hours. The
following monoclonal primary antibodies were used in the Western blot analysis:
anti-NOCA2 (Abcam), anti-glyceraldehyde 3-phosphate dehydrogenase (CST),
anti-Slug (Abcam), anti-N-cad (Abcam), anti-E-cad (Abcam), anti-Vimentin
(Abcam), anti-β-catenin (Abcam), and anti-TCF-8 (Abcam), overnight at 4 °C. The
membranes were washed 3 times with TBST (0.1% Tween-20) for 10 minutes, each at
room temperature. The signal was visualized by chemiluminescence kit.
