ALDH Activity Quantification Assay

ALDH activity was determined with the AldeRed ALDH detection assay (Merck Millipore) according to the manufacturer's instruction, which is also described in [53 (link)]. Briefly, verapamil was dissolved in PBS and added as a 1:100 dilution to the cells (final concentration 24.6 μg/ml). AldeRed 588-A was added in a 1:200 dilution to the verapamil treated cells for 30 min. Cells pre-treated with DEAB solution (dilution 1:100, final concentration 15 μM) were used as a control.

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Zimmermann L., Moldzio R., Vazdar K., Krewenka C, & Pohl E.E. (2016). Nutrient deprivation in neuroblastoma cells alters 4-hydroxynonenal-induced stress response. Oncotarget, 8(5), 8173-8188.

Publication 2016

AldeRed ALDH detection assay

Assay Cells Deab Dilution Verapamil

Corresponding Organization :

Other organizations : University of Veterinary Medicine Vienna, Rudjer Boskovic Institute

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Variable analysis

independent variables

Verapamil concentration (24.6 μg/ml)

dependent variables

ALDH activity

control variables

DEAB solution (15 μM)

controls

Positive control: Cells pre-treated with DEAB solution (dilution 1:100, final concentration 15 μM)
Negative control: Not explicitly mentioned

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