Epithelial-Mesenchymal Transition in Immortalized hSAECs

An immortalized primary human small airway epithelial cell (hSAEC) line was previously described.^{12 (link),14 (link)–18 (link)} The immortalized hSAECs were grown in SAGM small airway epithelial cell growth medium (Lonza, Walkersville, MD) in a humidified atmosphere of 5% CO₂. For induction of EMT, hSAECs were TGFβ stimulated for 14 d (10 ng/mL, PeproTech, Rocky Hill, NJ).^{14 (link)}

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Zhang J., Jamaluddin M., Zhang Y., Widen S.G., Sun H., Brasier A.R, & Zhao Y. (2019). Type II Epithelial-Mesenchymal Transition Upregulates Protein N‑Glycosylation To Maintain Proteostasis and Extracellular Matrix Production. Journal of proteome research, 18(9), 3447-3460.

Publication 2019

SAGM small airway epithelial cell growth medium TGFβ stimulated

Atmosphere Cell line Epithelial cell Growth medium Human Tgfβ

Corresponding Organization : University of Wisconsin–Madison

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Variable analysis

independent variables

TGF-β treatment (10 ng/mL)

dependent variables

Induction of epithelial-mesenchymal transition (EMT) in human small airway epithelial cells (hSAECs)

control variables

Culture conditions: SAGM small airway epithelial cell growth medium, humidified atmosphere of 5% CO2

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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