Blood samples were collected in Sarstedt S-Monovette K3 EDTA or BD Vacutainer K3 EDTA tube prior to bowel preparation for bowel colonoscopy (BLITZ study), or prior to large bowel surgery or neoadjuvant therapy (239 CRC cases from the clinical setting). The samples were transported to the laboratory while preserving a cold chain and were centrifuged at 2000–2500× g for 10 min, aliquoted and stored at −80°C until further use. Details on the SOPs have also been described previously.19 (link)
Protocol detail
Blood Sample Collection and Processing for CRC Research
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Corresponding Organization :
Other organizations : German Cancer Research Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Heidelberg University, National Center for Tumor Diseases
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Variable analysis
independent variables
- Blood sample collection method (Sarstedt S-Monovette K3 EDTA or BD Vacutainer K3 EDTA tube)
- Not explicitly mentioned
- Time of blood sample collection (prior to bowel preparation for bowel colonoscopy or prior to large bowel surgery or neoadjuvant therapy)
- Cold chain preservation during sample transportation
- Centrifugation at 2000–2500× g for 10 min
- Aliquoting and storage at -80°C
- Not explicitly mentioned
- Not explicitly mentioned
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