For in vivo trafficking and analysis of excretion levels, biotinylated GQDs were intraperitoneally infused. To determine the remaining amounts of GQDs, mesentery tissue was collected after mice were sacrificed. After the tissue was minced and homogenized, it was incubated with FITC-conjugated anti-biotin antibody (BD Biosciences). Fluorescence was assessed using flow cytometric analysis. In addition, urine samples were also collected at designated time points to investigate excretion as described in a previous report (14 (link)). The concentrations of the GQDs in urine samples were measured by the Quant*Tag Biotin Kit (Vector Laboratories Inc., Burlingame, CA, USA).
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In Vivo Trafficking and Excretion of Biotinylated Graphene Quantum Dots
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Corresponding Organization : Advanced Institute of Convergence Technology
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Variable analysis
independent variables
- Intraperitoneal infusion of biotinylated GQDs
- Remaining amounts of GQDs in mesentery tissue
- Excretion levels of GQDs in urine samples
- Not explicitly mentioned
- Positive control: Not mentioned
- Negative control: Not mentioned
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